, Sunnyvale, CA, USA). Cyanobacterial strains that did not lyse until the 12th day were defined as insusceptible. 1 N NaOH (50 μl) followed by absorbance measurement at 405 nm with a plate reader (VersaMax, Molecular Devices, Sunnyvale, CA). 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pm SpectraMax and VersaMax . Tested w/warranty. Then the medium was removed and 200 μL of DMSO was added to each well to solubilize the formazan crystals. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax microplate spectrophotometer provides rapid and sensitive measurements for a variety of analytes across a wide range of concentrations. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. While the cell covers about. pdf file (03 Dec 2022) Manufacturer: Molecular Devices; Category of Device: Laboratory Equipment, Measuring Instruments; Document: versamax Operator's Manual, File Type: PDF;Search for used versamax. 0 OD Kinetic Read Time: 9 Second Minimum Interval Temperature Uniformity ±0. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). The activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). P. After 4 h of incubation at 37 °C, culture medium was discarded and dimethyl sulfoxide (150 μL/well; Sigma) was inoculated to dissolve the precipitated formazan produced in viable cells. The rate of cell viability was calculated using the following formula:The conversion of NADP+ to NADPH was measured as absorbance (340 nm) on a microplate reader (VersaMax, Molecular Devices Co. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. 00 Regular price. Molecular Devices公司的VersaMax读 板机为光栅型单色器读板机，拥有灵活检 测优势，无需加配额外的滤光片。 可调波长，无需滤光片，灵活 VersaMax读板机使用光栅单色器来选择 波长，其结果是选择确切的检测波长应用 于每个实验。波长范围从340nm到 With the VersaMax microplate reader, you will never have to buy another filter. used. Patients with small intestinal neuroendocrine tumors (SI-NETs) frequently develop spread disease; however, the underlying molecular mechanisms of disease progression are not known and effective. The signal produced was proportional to the amount of analyte present and interpolated from the calibration curve presented on each plate. We would like to show you a description here but the site won’t allow us. Results of host range test of Mae-Yong924-1 against. The collaborative space brings customers and researchers into the lab to test automated workflows for organoid culturing and screening, with guidance from in-house. 00 Add to cart Molecular Devices Microplate Reader Spectramax 384 340PC $ 6,295. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. Experimental data analysis was performed with a one-way analysis of variance (Anova with Newman-Keuls post-test) in Prism 6. +49-89/96-05-88-0 fax +49-89/9-62-02-34-5 Japan Nihon Molecular Devices Osaka Tel. reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA) at 560 nm with background subtraction at 630 nm, based on which the corresponding cell viability was assessed. Filterless. 5°C @ 37°C Well-to. SpectraTest ABS1 Absorbance Validation Plate. Data were analyzed with GraphPad Prism. New and used Molecular Devices VersaMax's for sale on LabX. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmSpectraMax and VersaMax . Each sample has a discrete sample beam and reference beam so that each well is measured directly. S. 340‚ 850 nm. versamax, vmax, emax, uvmax, flexstation, softmax pro 5 gxp software, spectratest, molecular devices automation partners, gemini, lmax, assay protocols, data display, data analysis, assay. SoftMax® Pro Software Validation Package provides the most comprehensive documentation and tools available to validate GxP administrator features, software. Statistical analysisThe absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. 00 USD. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Find regional support. Molecular Devices Versamax Absorbance Microplate Reader BN 02970. ,, [28]. g. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. Bacterial growth was determined turbimetrically using the microplate ELISA reader VERSAmax (Molecular Devices). The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software. Search for used versamax. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The water extract afforded the greatest yield, and total phenolic and tannin. The VersaMax Absorbance Microplate Reader allows for selection of. Measurement of changes in BDNF production and release was done byMicroplate Readers. 4. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). 4. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. This manual is subject to change without notice. 0. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Sponsored. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. 1. molecular devices | 1311 orleans drive | sunnyvale, cA 94089 usA | email: info@moldev. g. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. The optical density (OD) of the mixture was measured at 490 nm with a microplate absorbance reader (VersaMax (Molecular Devices), Shanghai, China). Whether bacteria were partially or completely inactivated by bone cement. For more information, please visit us at With the VersaMax microplate reader, you will never have to buy another filter. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. Unit price / per . 99 + $59. Sterile AB medium and a mixture of Me 2 SO, methanol, and water (ratio v/v 1:1:8) served as negative controls. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 0%, 0-2. The absorbance at wavelength 450 nm was detected using a micro-plate reader (VersaMax, Molecular Devices, USA). After treatment, cells were collected, lysed with the lysis buffer. Related products. 4. We would like to show you a description here but the site won’t allow us. Ann Arbor, MI, USA). Select Update Driver. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Next, 100 μL of a reagent was added to an equal volume of cell culture medium present in each well. $495. to 11. 8). Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. Learn how to use SoftMax. Molecular aspects of color variation in luciferase emission have been the subject of numerous studies: various mechanisms have been proposed [6] and multicolor reporter proteins have been created and applied in cell-based. DISCLAIMER Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. 8), 40 μL of tyrosinase, and 40 μL of L-DOPA was put in each well. Cell-free AB broth (22) of an overnight culture of the acylhomoserine lactone-defective V. The absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. VersaMax ELISA Microplate Reader. Youmustensurethatthemeniscusiscomfortablyabove thelightbeaminstandardcuvettesandthatthesamplechamberinamicrocuvetteis Microplate Reader Capability > UV - Vis Absorbance. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. pneumoniae ATCC49619 was used as reference control strain as recommended (Clinical and Laboratory Standards Institute. The product formed was then transferred to 96 well plate and the absorbance was measured at 540 nm using microplate reader (VersaMax™, Molecular Devices, CA, USA). Molecular Devices also offers a mass spectrometry-based solution for food safety testingWe would like to show you a description here but the site won’t allow us. GABase was a mixture of γ-aminobutyrate glutamate amino transferase. 1 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 compliance to ensure data integrity. Bovine serum albumin was used as a reference. Add to Cart. The absorbance of each well was measured at λ = 490 nm with a VersaMax (Molecular Devices, USA) microplate reader. SpectraMax®, VersaMax, VMax. S. The IC 50 value was generated from the log dose-response curves for cells using the Graphpad Prism version 9 for Windows (Graphpad Software, La Jolla, CA, USA). Louis, USA) was used to. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. After freeze-drying, the. For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. For pinocytosis, cells in wells were washed in PBS and 5 mg/ml of HPTS dye (in PBS) added and incubated with gentle rocking at 30 °C for 120 min. (Seongnam, Korea). SpectraTest® Validation Package. Microplate Reader Capability > UV - Vis Absorbance. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. ¨We would like to show you a description here but the site won’t allow us. The absorbance was read at 570 nm using a spectrophotometer (VersaMax, Molecular Devices, San Jose, CA, USA). The average survival rates for cells exposed to dry. 2. The optical density (OD) was measured at 570 nm (with a reference at 690 nm) by UV-visible spectrophotometer (VersaMax, Molecular Devices, CA, USA) in triplicates. Antioxidant enzyme activityProtein concentration was determined from diluted crude venom and nanoparticle samples (1 mg/mL) in deionized water by BCA™ Protein Assay using a UV/V spectrophotometer (VersaMax, Molecular Devices, CA, USA) at a wavelength of 562 nm. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. A series of 44 excipients were evaluated at six different concentrations in triplicate. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. Myeloperoxidase AssayThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The cells were seeded on the bottom of 24-well plates with sterile glass coverslips. After three times washing, the reaction was colored by substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (T0440, Sigma), and stopped by 2 N HCl. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Cet appareil ISM est confomre à la norme NMB-001 du Canada. 8), 40 μL of tyrosinase, and 40 μL of L-DOPA were put in each well. ®SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 4. HepG2 cells were seeded into 6-well plates. 90 shipping. $1,749. Table of Contents. This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. used. SoftMax® Pro 5 Software from Molecular Devices offers extensive data capture, analysis, and reduction capabilities for its absorbance, luminescence,. (Molecular Devices, Sunnyvale, CA). 00. Cell migration assay. Add to Cart. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. With optimized reagents, option to operate the. The lysozyme concentration of a sample was determined using a standard calibration curve made of a series of known lysozyme concentrations. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. , Sunnyvale, CA, USA). DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. Cell viability was analyzed using a cell counting kit assay (Dojindo). 7. printed in u. Molecular Devices SoftMax Pro 5. +86-10-6410-8669 fax +86-10-6410-8601 Molecular Devices Shanghai Tel. 0A-Sunnyvale, CA-Made in USASpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The optical density (OD) was measured at 570 nm (with a reference at 690 nm) by UV-visible spectrophotometer (VersaMax, Molecular Devices, CA, USA) in triplicates. Ensure data accuracy of your reader. com we know that an user's manual may be needed at any time. Spectrophotometers. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). The optical density of each well at 490 nm was then measured using a microplate spectrophotometer system (VersaMax; Molecular Devices, Sunnyvale, CA, USA). +1 800-635-5577. Manufacturer: Molecular Devices; Includes PC Workstation w/ Softmax Pro Software Wavelength Range (nm): 340–850 Wavelength Accuracy: < ±0. 635. s. SoftMax® Pro 7. Wavelength Range. The SpectraTest® ABS1 Absorbance Validation. The absorbance of the mixture was measured with a microplate reader (VERSAmax™, Molecular device, Sunnyvale, USA) at wavelength of 498 nm until absorbance was saturated. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Cells were treated with 150 μl of DMSO and absorbance was quantified in 540 nm using the microplate spectrophotometer system (VersaMax, Molecular Devices, Sunnyvale, CA, USA). Dual monochromatic, three modes detection, UV Vis Abs, Fluor, and Lum, cuvette. Plates values were acquired using a fluorescence microplate reader (570 nm; Versamax, Molecular Devices, San Jose, CA, United States). Table of Contents. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. The TBARS content was calculated according to the extinction coefficient as 155 mM −1 cm −1. Versamax Microplate Reader dibangun di sekitar monokromator kisi. Absorbance was determined at 490 nm on a microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). 0 OD. measured over 60min using a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA) at a wavelength of 405nm. +44-118-944-8000. 2. 0A 2 Lines Fused, unplug before ser. 6/10 #0120-1330d. Tristar 3 Multimode Microplate Reader. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 4 0112-0126B SafetyInformation InformationaboutthesafeuseoftheinstrumentfromMolecularDevices®includesan Get the most out of SoftMax Pro. Four wash/rinse bottles are included and configurable for both 96- and 384. Table 1. Used. #VERSAMAX) SoftMax Pro GxP Software version 5. 5% NP-40) supplemented with protease and phosphatase inhibitor cocktail (GenDepot, Baker, TX, USA). Florescence intensity was monitored on a Gemini XPS fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA) set for 488 nm excitation and 520 nm emission. The activity was measured at 40 °C following o-nitrophenol (ONP) release at 405 nm using a microplate reader (Versamax, Molecular Devices, San Jose, CA, USA). GABA concentrations in samples were calculated from calibration curves using the standard solutions. The Indocyanine green (ICG, Sigma-Aldrich) assay measures the liver depuration capacity . Access all the resources, tools and information you need to stay productive. repurposing of drug molecules already approved, or in clinical trials, for other viruses. and California VERSAMAX Tunable Microplate Reader 100-240V - 4A 50-60 HZ T4. It also integrates with the. Readings were taken every 15 s at 490 nm and the results were analysed using SOFT-MaxPro 4. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. reader (VersaMax, Molecular devices, San Jose, CA, USA). 03 billion doses of. ¨ Molecular Devices is a life-science technology innovator Our integrated hardware and software solutions enable automated workflows—from start to finish—for any research application. Monday to Friday 7am - 5pm Pacific Time. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Ensure data accuracy of your reader. S. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. MOLECULAR DEVICES VersaMax Reader: Model #: VersaMax: Serial #: N/A: Year Built: N/A: Location: California: Price: $3,800. The RT-qPCR analysis was performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). Windows Home Editions are not supported. . We would like to show you a description here but the site won’t allow us. Bimolecular fluorescence complementation (BiFC) systemMolecular Devices Versamax Tunable Microplate Reader is available from The Lab World Group. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Printed in U. Nude mice were obtained from Orient Bio Inc. US HEADQUARTERS. DISCLAIMER Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. It covers everything from installation, configuration, data acquisition, data analysis, curve fitting, to reporting and troubleshooting. Spectranet customer portal. 0, 0. The colour change of the MTS solution was measured at 490 nm by using a tunable microplate reader (VersaMax Molecular Devices, USA). Molecular Devices VersaMax Absorbance (UV/Vis) Elisa Microplate Reader For Sale for Plate Reader is complete with computer, SoftMa. MDC instruments including: SpectraMax®, VersaMax. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. All the experiments were conducted at. We would like to show you a description here but the site won’t allow us. , USA) at 490 nm. Taking into account the price differences between each seller and each country. 0%, 0-2. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. We would like to show you a description here but the site won’t allow us. This document is provided to customers who have purchased Molecular Devices, Inc. SoftMax Pro. The Versamax Microplate Reader is built around a grating monochromator. Softmax Pro 소프트웨어가 포함된 PC 워크스테이션 포함. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. All samples were measured in triplicate. 99 + $91. Absor-bance of the solution was measured using a micro plate Reader (VersaMax, Molecular Devices) at 570 nm. The in vitro differentiation of BMDMs. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. Finally, the absorbance was determined at 490 nm on a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA). This version is compatible with the following instruments: SpectraMax® i3x Multi-Mode Detection Platform. The absorbance was measured at 490 nm using a VersaMax ELISA reader (Molecular Devices, Sunnyvale, California, United States). For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. SpectraMax® Microplate Reader User Manual Download Page. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. The culture supernatant of cells (25 μl) was mixed with 225 μl of DMMB dye solution and the absorbance of solution mixture was measured at 525 nm with a spectorophotometer (VERSAmax; Molecular Devices Japan KK, Tokyo, Japan). The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Phosphodiesterase isozymes: molecular targets for novel antiasthma agents. Model: VERSAmax Tunable Microplate Reader. To update the driver. This technical note is designed to provide information . The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). Molecular devices Versa Max. The. 45% of MTT reagent in cell culture media was substituted and incubated for 4 h before adding 100 μL of 100× DMSO. 9000-0756. Molecular Devices Corporation. The absorbance was measured at 540 nm using a hybrid microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). Tristar 3 Multimode Microplate Reader. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. The wells were visualized using o-phenylenediamine (OPD), and the absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 490 nm. ® The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 0A Sunnyvale, CA Made in USA The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. Also see: SoftMax® Pro Microplate Software User Manual Download Page. Thus, the molecular mechanism promoting hepatic lipid accumulation in obesity is of biomedical and public health interest. Fluorescence-activated cell-sorting analysis For analysis of DC surface molecules, 5×10 5 DCs were treated with soluble OVA (20 μg/mL), PLGA NPs (20 μg/mL OVA), PEI-coated PLGA NPs (20 μg/mL OVA), or 1 μg/mL. Windows 10 – 2004/20H2/21H1/21H2. recombinant proteins, including monoclonal antibodies. The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). used. RT-qPCR. (VersaMax Molecular Devices, Sunnyvale, CA). The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 키네틱 읽기 시간 : 최소 9초 간격. Proliferation of the released daughter cells was monitored at a wavelength of 578 nm online by a microplate reader (VersaMax; Molecular Devices, Sunnyvale, Calif. 滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software, SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The color was allowed to develop for 20 min in dark and the reaction was stopped with 2. (JC-1) (Molecular Probes), respectively. 2. With optimized reagents, option to operate the. 2 and 648. This reliable model allows for. The reaction was stopped with 100 µL of H 2 SO 4 1 M and quantified in a spectrophotometer (VERSAmax, Molecular Devices, San Jose, CA, USA) at 450 nm. A. The percentage of cytotoxicity was calculated using the following formula: cytotoxicity (%) = [1 − (At/As) × 100], where At and As refer to the absorbance of a tested. The colorimetric reaction was read using an automated ELISA microplate reader (Versamax, Molecular Devices, USA) at 492 nm. The Organoid Innovation Center at Molecular Devices combines cutting-edge technologies with novel research methods to address key challenges of scaling complex 3D biological models. 0A-Sunnyvale, CA-Made in USA SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. After washing in PBS at least 4 times to remove all extracellular dye, samples were fixed using 4 % PFA/PBS for. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. Wavelength Accuracy. 23,225, Thermo Fisher Scientific). The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. Resveratrol (1 µ M) was expected to produce a sufficient effect on ER-stress and ROS production and was applied for subsequent analysis; a dose-dependent effect was also investigated by using 1 and. The final mixtures were allowed to react at 25 °C in the dark for 5 min. 3, Molecular Devices). Then the absorbance at 570 nm was measured using a microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA). VersaMax™ Tunable Microplate Reader, Molecular Devices. SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PM At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. 5°C @ 37 °C W. The cytokine concentration was calculated from the standard curve using seven-parameter curve fitting software (SOFTmaxPro 5. The VersaMaxTM Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). the plate reader (model VERSAmax, Molecular Devices, USA) at 515 nm (A515). After 30-min incubation, the absorbance was measured at 405 nm wavelength using a spectrophotometer device (VersaMax Molecular Devices, Sunnyvale, CA, USA). To test if sodium arsenite and dimethylarsenic acid could stimulate apoptosis in 3 cells, oc cell cycle redistribution was studied using flow cytometry with propidium iodide staining. 0. Am J Respir Crit Care Med 1998; 157: 351–370. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). SpectraMax Plus384, 190, 340PC384, and VersaMax User Guide Molecular Devices User Guide SpectraMax. This technical note is designed to provide information . # SMP54-GXP- 10UL) Pyrochrome Assay. The value for non-specific absorption at 600 nm was subtracted. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. TECHNICAL NOTE. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Select the Driver tab. One microgram of RNA was. 3 Methods 1. The plates were immediately measured at 405 nm for 6 min using a microtiter plate reader (VersaMax; Molecular Devices, Sunnyvale). Our mission: find your user guide or user's manual as quickly as possible. The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements allowing for selection of up to two wavelengths at a time. HL-60 cells were plated at a density of 3×105 cells/well on 6-well plates. All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. The SpectraTest® ABS1 Absorbance. After incubation, the absorbance of the plate was measured at 450 nm using a microplate reader (VERSAmax™, Molecular Devices Corp. The amount of MTT reduction was measured immediately by detecting absorbance in a microplate reader (Versamax, Molecular Devices) at a wavelength of 570 nm and a reference wavelength. 1 mL. In brief, mature 3T3-L1 cells were washed with DPBS, fixed with 10% formalin for 1 h at room temperature, washed once with 60%. Molecular Devices Spectramax Plate Reader. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Proliferation was assessed by bromodeoxyuridine (BrdU) incorporation (Cell Signaling Technologies). Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. SpectraMax and VersaMax Plate Readers Operator s Manual -- 0112-0126 Rev A Molecular De. from Molecular Devices Corporation. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. MDC instruments including: SpectraMax®, VersaMax. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the device, as well as examples of applications and. The absorbance value (OD) was measured at 492 nm with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA). Briefly, MTT (200 μl, 2 mg/ml) was added to the wells and incubated for 4 h at 37°C. With a wavelength. , VersaMax; Molecular Devices) Incubator for cell culture. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 68 $. If the computer is connected to the Internet: Select Search Automatically for. The assay was performed in 96-well microtiter plates in duplicate, and the absorbance was read at 405 nm with 620 nm as the reference wavelength using a microplate reader (VersaMax, Molecular Devices, Boston, Massachusetts). The senescence-associated β -galactosidase activity (SA- β -gal) was measured using β -galactosidase Assay Kit following manufacturer's protocol. The absorbance was assumed to be 100% for the control group. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. Statistical Analysis. The absorbance was measured at 405 nm using a plate reader (VersaMax, Molecular Devices) (N = 3). Regular price $ 0. Molecular Devices Corporation. We would like to show you a description here but the site won’t allow us. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. The absorbance was measured at 570 nm and. Briefly, 100 μl of cell culture medium was mixed with 100 μl of Griess reagent [equal volumes of 1% (w/v) sulfanilamide in 5% (v/v) phosphoric acid and 0. The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can. No accessories are needed and SoftMax® Pro Software. Wavelength Bandwidth °¤ 2. S. If you are looking for a comprehensive user guide for SoftMax Pro 7. 1 software, the leading microplate data acquisition and analysis software, you can download this PDF file from Molecular Devices. The absorbance of reaction mixtures was read at 475 nm using a microplate reader (VERSAMax, Molecular Devices) to determine the quantity of dopachrome produced. 0A Sunnyvale, CA Made in USA Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. TheMolecular Devices VersaMax Microplate Reader (ZB64) . Order Now. Regular price $ 0. For determination of the optimal temperature, EfmTDC was incubated in 100 mM sodium acetate buffer (pH 5.